PIK3CA and urinary bladder cancer: Another recent study using seven human bladder cancer cell lines in a 3D high-throughput screening platform showed that the combination of AKT inhibitor (AZD5363) with a dual PI3K/mTOR inhibitor (NVP-BEZ235) or an mTOR inhibitor (AZD2014) exhibited synergistic effects on cell viability and colony formation [152].