HOVs-cyst-1 cells did not develop any colonies, whereas the double KRAS and PIK3CA mutant HOVs-cyst-1 cells, as well as HOVs-cyst-1 cells, with either KRAS or PIK3CA mutant, showed a small number of colonies compared with the SKOV3 cells (Figure 4a,b). The gene discussed is KRAS; the disease is cyst.