PIK3CA and cyst: Furthermore, analysis of the migration ability of the cells via an in vitro wound-healing assay showed that immortalized HOVs-cyst-1 cells, with both KRAS and PIK3CA mutations, had significantly (p < 0.01) higher migration abilities than HOVs-cyst-1 cells, with either KRAS or PIK3CA mutation (Figure 3b,c).