Although the remaining tumor cells (%live cells) were similar for both SH-SY5Y and LAN-1 cells as a function of thermal dose (Figure 6A), ICD marker expression was higher in SH-SY5Y cells than LAN-1 cells as measured by decreased intracellular ATP (Figure 6B), increased extracellular HMGB1 (Figure 6C), and higher calreticulin expression (Figure 6D) at each thermal dose. Here, HMGB1 is linked to neoplasm.