Importantly, we have already shown in vivo the strong anti-leukemic activity of 8-Cl-Ado in combination with VEN in an orthotopic mouse model of AML, using the fast-growing FLT3-ITD-positive AML cell line MV4-11 [19], but the mechanistic basis for this synergism remains to be fully elucidated. This evidence concerns the gene FLT3 and acute myeloid leukemia.