NES and glioblastoma: In detail, when counting nestin immuno-gold particles within the whole cell (Figure 14E), cytosol (Figure 14F), nucleus (Figure 14G), and the relative intracellular distributions of the protein across the cytosol and nucleus (Figure 14H), rapamycin-induced suppression of the protein within astrocytes co-cultured with GBM cells is similar within all compartments.