Cui et al. (2019) focused on using a third-generation CD8 hinge, CD28 transmembrane intracellular domain, 4-1BB, CD3ζ-based anti-CAIX scFv (type not described) CAR T cells maintained in culture with IL2 in an orthotopic glioblastoma model by intracranial inoculation with 100,000 U251 cells. Here, CA9 is linked to glioblastoma.