In the present study, we investigated the possibility of using human brain organoids (hBOs) as a potential stem cell therapy platform by evaluating the neuroprotective effect of hNTSCs against Aβ-induced toxicity in hBO culture and then analyzing the role of osteopontin (OPN) as a secretory protein involved in the therapeutic potential of hNTSCs against Aβ toxicity in hBOs and a 5 × FAD transgenic mouse model of AD. The gene discussed is SPP1; the disease is Alzheimer disease.