Here, the HFD-induced hepatocellular hypertrophy and steatosis were suppressed by the metformin combination with LD, more so than metformin alone, together with reduced levels of blood biochemical parameters for assessing the severity of NAFLD (ALP, ALT, AST, GGT, LDH, glucose, insulin, triglyceride, and total/LDL cholesterol). This evidence concerns the gene INS and steatosis.