Subsequently, site-specific mutant luciferase constructs of AP-1 and NF-κB in the IL-8 promoter were transiently transfected into human colorectal HCT-116 cells to confirm the role of AP-1 and NF-κB in LCA-stimulated IL-8 transcription. This evidence concerns the gene NFKB1 and Leber congenital amaurosis.