Similarly, the suppression of LCA-stimulated IL-8 expression with BAY or SN50 (an NF-κB p65 nuclear translocation inhibitor) treatment is illustrated in Figure 2D. Piperine partially blocked NF-κB, both at the transcriptional and protein levels, and SN50 significantly inhibited LCA-induced IL-8 expression (Figure 2F,H). Here, NFKB1 is linked to Leber congenital amaurosis.