Subsequent inhibitor studies confirmed that EGFR, Src, and AKT signaling pathways were involved in the suppression of LCA-stimulated IL-8 expression by piperine in HCT-116 cells, using RT-PCR (Figure 3G) and the luciferase activity assay (Figure 3H). The gene discussed is AKT1; the disease is Leber congenital amaurosis.