Notably, chlorogenic acid showed a protective effect from Con A-induced hepatitis in mice through inhibition of Toll-like receptor 4 signaling, alleviation of infiltration, and reduction of pro-inflammatory cytokines production [34], while 3,5-di-CQA exhibited the potent chondroprotective and anti-nociceptive activities in an animal model, suggesting that this compound affected osteoarthritis based on its strong anti-inflammatory and antioxidant potentials [35]. The gene discussed is TLR4; the disease is osteoarthritis.