VIM and Gliosis: Leukostasis assay: number of adherent leukocytesH&E staining: observation of general retinal and the optic nerve head morphologyNeurodegeneration: number of RGC (RBPMS) and cholinergic amacrine cell (ChAT); Inflammation and Gliosis: number of macrophages (F4/80); and Muller cell (vimentin)