NMC is diagnosed by a NUT immunohistochemical (IHC) assay that stains the nuclei of NMC cells and has a specificity of 100% and sensitivity of 87% for the diagnosis of NMC, using monoclonal rabbit antibody to NUT (clone C52B1, Cell Signalling Technology, Danvers, MA, USA). The gene discussed is NUTM1; the disease is nut midline carcinoma.