When the OS of patients with HBV-related HCC was plotted according to the use of NAs, significant improvement was noted in patients who used NAs compared to those who did not (Fig 3B; adjusted HR 0.72, 95% CI 0.64–0.80) after adjusting for age, AFP, ECOG PS, CP class, and mUICC stage. This evidence concerns the gene AFP and hepatocellular carcinoma.