Tomograms for this application are collected on INS-1E cells (a rat insulinoma cell line) [38], treated with various combinations of 25 mM glucose, 10 nM Exendin-4 (or Ex-4, a glucagon-like peptide-1 receptor agonist that enhances glucose stimulated insulin secretion), and 30 μM NN414 treatment (a selective SUR1/Kir6.2 potassium channel opener that inhibits insulin secretion). The gene discussed is INS; the disease is pancreatic insulinoma.