The purpose of this study was to investigate the antibody responses induced by LSA and recombinant kinetoplastid membrane protein 11 (rKMP11) in serum samples from healthy and sick dogs by in house enzyme-linked immunosorbent assay (ELISA) and the effects of LSA and rKMP11 antigens on the ex vivo induction of specific IFN-γ cytokine responses in whole blood from dogs with different states of infection. This evidence concerns the gene IFNG and infection.