Consequently, an increase in the expression of tumor necrosis factor-α (TNFα) and Gas dermin D (Gsdmd), cleavage of caspase-1, phosphorylation of ASC (Pycard) and ERK convincingly pointed towards activation of pyroptosis, formation of inflammasomes and a biologically delayed response of transitioning from S-AKI to CKD. This evidence concerns the gene PYCARD and chronic kidney disease.