In this study, a recombinant adeno-associated viral (rAAV) -mediated shRNA was constructed to knockdown SLC1A1/EAAT3 in adult mice, and an LPS-induced cognitive impairment model (POCD animal model) was established to identify the function of EAAT3 during the development of POCD and its underlying mechanisms. This evidence concerns the gene SLC1A1 and Cognitive impairment.