TLR2 and infection: In Leptospira, it has been proven that the LipL32 stimulated inflammatory responses through TLR2 and the calcium-binding cluster (including Asp132, Thr133, Asp164, Asp165, and Tyr178) of LipL32 that could regulate the affinity of LipL32 and TLR2 upon infection of Leptospira [7,42].