Limitations include radiation exposure to patients and staff, strong legislative control, limited radiotracer availability, expensive detectors and radiotracers, strong initial signals causing “shine through” (a particular concern in bladder cancer as the injection site in the bladder mucosa will be near many of the perivesical LNs of interest), long preparation times, limited half-life of 99mTc (6 h), allergies to blue dyes, viral transmission (through the albumin-based 99mTc colloids as this is derived from human blood), and dependency on nuclear medicine units [42]. This evidence concerns the gene ALB and urinary bladder carcinoma.