To verify our observation from reporter assays, we then performed quantitative reverse transcription PCR (qRT-PCR) analysis to measure the transcriptional levels of antiviral genes, such as IFNB1, IFIT1, and CXCL10, in human macrophage THP-1 cells with or without PCBP2 overexpression following the infection of HSV-1. Here, IFNB1 is linked to infection.