Our data demonstrated that GAS5 enhances NOTCH2 expression by sponging miR-29a-3p and promotes NAFLD progression by targeting miR-29a-3p, suggesting that miR-29a-3p is involved in GAS5-modulated NAFLD and supporting the important role of miR-29a-3p in NAFLD as indicated in previous studies. This evidence concerns the gene NOTCH2 and metabolic dysfunction-associated steatotic liver disease.