To investigate the whether HBX is involved in HMMR-AS1 regulation in HBV-HCC, we transfected HBV-positive HCC cells (Huh7-1.3 and HepG2.2.15) with siRNA targeting HBX to reduce HBX level (Figure 2(a)). The gene discussed is HMMR; the disease is hepatocellular carcinoma.