Thus, in the present study, we inhibited the expression of M3 receptor by siRNA to observe whether M3 receptor participated in the suppressive effect of HE on CKD, identifying that M3 receptor silencing improved the proliferation, fibrosis, and downstream proteins of TGF-β in TGF-β-induced NRK-49 F cells. The gene discussed is TGFB1; the disease is hereditary elliptocytosis.