SHH and neoplasm: To address this, tumor cells from Lcre;NICD1 mice were treated with a recombinant amino-terminal fragment of SHH (ShhN) and a small molecule Inhibitor of Mastermind Recruitment 1 (IMR-1, or IMR-1A) to block the recruitment of Mastermind-like protein 1 (MAML1) to the NOTCH transcriptional complex, or infected with viruses expressing GFP fused to dominant negative MAML1 (dnMAML1) that disrupts the complex [48, 49].