In addition, we have previously adapted a technique developed to investigate the binding of ligands to neurotransmitters in mouse and rat brain neuronal plasma membrane receptor preparations (MRPs)19 as an in vitro system for identifying interactions of YF strain FNV, Japanese encephalitis, West Nile and tick-borne encephalitis viruses to MRPs derived from mouse and monkey brain20–24. The gene discussed is MRPS7; the disease is Japanese encephalitis.