Third, we use a nested case-control study to detect GR, MR, EPAC, and DR protein expression and methylation of the promoter regions for GR (NR3C1 and NR3C2), MR (MTNR1A and MTNR1B), EPAC (RASGRF1 and RASGRF2), and DR (DRD1 and DRD2) genes in the placenta, to further analyze the relationship between maternal emotional or sleep conditions and infant sleep disorder. The gene discussed is RASGRF2; the disease is sleep disorder.