Here, we found that the MFR and AJN β-ecdysterone peak was very small (Figures 1B–D), additionally, β-ecdysterone exhibited more cellular cytotoxicity in normal MDBK cells than prostate cancer PC and DU145 cells (Figure 1E), suggesting that β-ecdysterone has no toxic effects on cancer cells. This evidence concerns the gene PPIB and Familial prostate cancer.