INS and diabetes mellitus: Wistar rats were used as controls and HHT rats were used as obese prediabetic model and subject to empagliflozin. With treatment, Fetuin-A decrease alongside with improved insulin sensitivity and decrease inflammation (51).Non-obese TLR knockout mouse is a prediabetic model by TLR-4 deficiency diabetes-promoted. Fetuin-A is independent of TLR-4 expression but can act as an adapter in the FFA-TLR-4 interaction (52).In a type 1 non-obese diabetic mouse, a prediabetic state was already found in the liver. The transition to a diabetic state was accompanied by increased levels of Fetuin-A (53).