It is also essential for cardiac septation.[41, 42, 43] TBX5 inactivation causes cardiac septal defects in animal models.[41, 42, 44] In humans, mutations in TBX5 are associated with CHD, including ASD and VSD.[45, 46, 47, 48, 49] Based on these findings, the appearance of high leucine level‐correlated CHD phenotypes in the present study could be explained by the inactivation of TBX5. Here, TBX5 is linked to coronary artery disorder.