TBX5 and coronary artery disorder: Using a Sirt3 knockout mouse model, we showed that in embryonic hearts of Sirt3 homozygous knockout mice, the K‐Leu levels of total protein and TBX5 and TBX5 K339 site were significantly increased (Figure 4O), nuclear localization of TBX5 was decreased (Figure 4P), expression of TBX5 downstream targets was reduced (Figure 4O), and CHD incidence was increased (Figure 4Q) as compared with those observed in wild type mice.