CD8A and metastatic neoplasm: Unsupervised clustering of concatenated viable CD8+ and CD4eff T cells in PBMC, normal tissue, primary, and metastatic tumors revealed six T-cell subpopulations with 1,000 cell counts and more (Fig. 2B), with clusters highlighting PBMC (red box) in CD8+ (Pop3 and Pop4; Fig. 2B) and CD4eff (Pop0 and Pop4; Fig. 2B).