We found that YTHDF1 depletion evidently ameliorated the acidification of the extracellular microenvironment for hypoxic breast cancer cells, accompanied with significantly reduced glucose consumption and lactate production levels (Supplementary Fig. S5A–D; Original western blot data 10), suggesting that YTHDF1 knockdown inhibited the glycolytic metabolism in breast cancer cells. The gene discussed is YTHDF1; the disease is breast cancer.