Meanwhile, CCK8 assay showed that transfection of miR-16-5p mimics significantly inhibited the proliferation of breast cancer cells, while the combined treatment of miR-16-5p with the treatment with miR-16-5p inhibitor or YTHDF1-expressing plasmids alleviated the miR-16-5p-mediated inhibitory effect (Fig. 4C, D). Here, YTHDF1 is linked to breast cancer.