CD274 and neoplasm: In fact, the cut-off used for positivity ranged from 1% of tumor cells staining for PD-L1 [52, 67, 69] to 25% [62]; other studies considered a combined analysis of tumor cell and macrophage staining [57], CPS-related criteria [68], or an immunoreactive scoring system, accounting for both percentage of immunoreactive cells and staining intensity [51, 64].