2021). This will give insight into the dynamic immune cell attack on beta cells, including the role and fate of the insulin-secretory granules, and can thereby aid in the design of better approaches tailored to type 1 diabetes treatment. To set up and test the newly designed algorithm, tomograms from healthy pancreatic islets from C57BL/6J mice were used and compared with islets from non-obese diabetic mice (NOD/ShiLtJ), which are widely used in type 1 diabetic research (Mathews et al. 2015). Here, INS is linked to type 1 diabetes mellitus.