AKT1 and colonic neoplasm: To point out whether the oncogenic roles of patient derived PDK1 mutations due to elevating the AKT activity, we depleted AKT1 in either Type I or Type II mutant PDK1-expressing colon cancer cells (Supplementary Fig. 9a), and observed that knockdown AKT1 markedly compromised the oncogenic roles of mutant PDK1 in promoting colony formation (Supplementary Fig. 9b, c).