Cell growth was assessed in plate colony formation assay and cell counting kit-8 (CCK8) assay and we found MYH9 knockdown significantly inhibited the proliferation (Fig. 3c, 3d), while MYH9 overexpression promoted the proliferation of ccRCC cells (Figs. 3d, 4c). The gene discussed is MYH9; the disease is nonpapillary renal cell carcinoma.