To further confirm the role of FGF4 in GDM, recombinant FGF4 were engineered, expressed and purified to examine whether FGF4 replenishment could regulate the adverse metabolic phenotypes of HFD induce gestational diabetic mice and protect fetus or not, GTT (glucose tolerant test) was performed since glucose intolerance is one of the well-defined characteristics of GDM. Here, FGF4 is linked to gestational diabetes.