Recently, several studies showed that tertiary lymphoid structures and B cell facilitate immunotherapeutic responses in melanoma, sarcoma and breast cancer.47, 48, 49, 50 Here, we showed that a major group of B cells with a CD43−IgD+ expression pattern was excluded in the immunosuppressive stages, concordant with CD4+ and CD8+ effector T cells, whereas the opposite was observed in the CD43+ IgD− B cell group. This evidence concerns the gene SPN and melanoma.