We observed that neither Tim-3 blockade nor PD-L1 blockade significantly augmented recognition of DCs pulsed with NY-ESO-1 protein or NY-ESO-1+ melanoma cell lysates by NY-ESO-1–specific CD8+ T cell clones, suggesting that Tim-3 blockade does not significantly increase DC crosspresentation capability. This evidence concerns the gene CD274 and melanoma.