To this end, DCs prepared from 1 HLA-A2+ HD were pulsed with NY-ESO-1 protein, LAGE-1 ORF2 protein as control, HLA-A2–restricted peptide NY-ESO-1 157-165, or cell lysates from 2 melanoma cell lines (NY-ESO-1+ MEL 285 or NY-ESO-1– MEL 136) as previously reported (33). This evidence concerns the gene CTAG2 and melanoma.