To confirm the relevance of these findings, PD-1+Tim-3– and PD-1+Tim-3+ CD8+ TILs were sorted from single-cell suspensions obtained from MM, labeled with PKH67, and coincubated 30 minutes with PKH26+ autologous CD45+CD3– cells and IgG control, aTim-3.18, or aPD-1 mAbs before flow cytometry and ImageStream (Figure 4C and Supplemental Figure 3B). This evidence concerns the gene PDCD1 and Miyoshi myopathy.