CDH5 and vascular disorder: To further address the underlying molecular functions of Ngbr in the vasculopathy at the postnatal and adult stage, we conducted EC-specific Ngbr knockout in Cdh5-CreERT2Ngbrfl/fl mice (hereafter termed NgbrECKO) by intraperitoneal injection of tamoxifen at both the postnatal stage (P1) and the adult stage (8–12 weeks), as illustrated in Figure 1A. Ngbrfl/fl mice injected with tamoxifen served as littermate controls (hereafter referred to as Ngbrfl/fl).