Specimens were interrogated using a 7-color multiplex immunofluorescence panel that included lineage markers CD3 (T cells), CD68 (monocyte-derived cells), CD11c (APCs); the CD163 macrophage phenotype marker; immunosuppressive p-STAT3; and tumor cells markers, GFAP (glioma) and AE1/E2 (lung brain metastasis). Here, GFAP is linked to central nervous system cancer.