For secondary syphilis, polA and Tpp47 reached 209 copies/mL (22 to 649 copies/mL) and 253 copies/mL (29.15 to 1,029 copies/mL), respectively, in urine sediment; these values are significantly higher than those in plasma (19.8 copies/mL [IQR, 0 to 37.4 copies/mL] versus 19.8 copies/mL [IQR, 0 to 43.45 copies/mL]) (P < 0.05 in both). The gene discussed is POLA1; the disease is syphilis.