As additional control, we analyzed secretion of transfected His6/myc-tagged PAUF (pancreatic adenocarcinoma up-regulated factor), a cargo of CARTS (carriers of the TGN to the cell surface) that bypass membranes related to Vps26 or Rab9a function (Wakana et al, 2012). Here, RAB9A is linked to pancreatic adenocarcinoma.