By observing the static location changes in the SOX9-positive rosette formation from fibrosis to cirrhosis, we found that the SOX9-positive rosette structure showed a gradual increase in the SOX9 expression level and changed to ductular-like structure from the periportal parenchyma to the portal region, which might imply the possibility that LPLCs differentiate to RDCs during cholestasis progression. The gene discussed is SOX9; the disease is cholestasis.