Importantly, inhibition of IDH2 by AGI-6780 exhibited potent cytotoxic effect on primary leukemia cells isolated from AML patients with wt-IDH2, while the same concentrations of AGI-6780 displayed minimum toxicity in normal human bone marrow cells using MTS assay (Fig. 8e) and flow cytometry analysis (Fig. 8f; Additional file 3: Fig. S8c, d). This evidence concerns the gene IDH2 and leukemia.