Since LAGE3 positively promotes the proliferation of HCC cells and breast cancer cells [9, 11], our study involved CCK8 assay, BrdU assay, and cell cycle assay to explore the LAGE3 knockdown effects on the proliferation of HCC cells, and it was observed that the cell proliferation rate of the LAGE3-siRNA group was extraordinarily reduced in SMCC-7721 and Huh-7 cell lines when compared with the control group (Fig. 3A, B). Here, LAGE3 is linked to hepatocellular carcinoma.