ENG and neoplasm: To create T cells secreting αCD133-αCD3 engager specific to the CD133 antigen on tumor cells and CD3 on T cells, we first created a lentiviral plasmid construct containing cDNA encoding the αCD133-αCD3 engager protein (pCDH.αCD133-αCD3-ENG) by restriction endonuclease cutting and cloning.