The surface expression of CD133 in CCA cells was examined by flow cytometry (S2 Fig) [27]; the CD133-expressing KKU-100 and KKU-213A cells served as tumor targets for T cells secreting αCD133-αCD3 engager, compared to low CD133-expressing MMNK-1 cholangiocytes. The gene discussed is PROM1; the disease is neoplasm.