In addition, the mRNA expression of ANP, BNP, and β-MHC mRNA in TAC group markedly increased compared to sham group, and the mRNA expression of ANP, BNP, and β-MHC mRNA in Trim27-KO TAC group significantly decreased compared to WT TAC group (Figure 3(c)). This evidence concerns the gene TRIM27 and persistent truncus arteriosus.