Our data also proved that nesfatin-1 levels in prediabetes were obviously higher than those in T2DM but no significant difference when compared to those in the healthy, which is the first delineation, despite the fact that others demonstrated mildly lower nesfatin-1 levels in impaired fasting glycemia and impaired glucose tolerance in contrast to those in the healthy group (21). This evidence concerns the gene NUCB2 and type 2 diabetes mellitus.