To further characterize the molecular differences between the RNA based subtypes, we analyzed the expression of 23 regulatory genes associated with bladder cancer (41–47), including the steroid hormone receptors ESR1/2, AR, and PGR, the nuclear receptors PPARG, three RARs (A/B/G), and three RXRs (A/B/G), the receptor tyrosine kinases ERBB2/3 and FGFR1/3, and the transcription factors FOXA1, FOXM1, GATA3/6, HIF1A, KLF4, STAT3, and TP63, We found significant differences in the expression of most regulators between Cluster 2 and the other two m6A subtypes. Here, ESR1 is linked to urinary bladder cancer.