To further verify the role of Mst1/2 in the suppression of hepatoma cell proliferation caused by α-hederin, HepG2 and SMMC-7721 cells were treated with Mst1/2 inhibitor XMU-MP-1 (3 μmol) for 3 h or 6 h before exposure to α-hederin. This evidence concerns the gene MST1 and hepatocellular carcinoma.